Evaluation of <i>Chilo suppressalis</i> resistance and analysis of <i>CRY1C</i> expression in transgenic rice

The insect resistance of Bacillus thuringiensis (Bt) transgenic rice (Oryza sativa L.) is mainly determined by the transcription CRY1C gene and translation process Cry1C protein. With this in mind, we analyzed expression protein content a Bt insect-resistant restorer line its F1 hybrids, evaluated to Chilo suppressalis various lines rice, 50% lethal concentration (LC50) for C. suppressalis. In four lines, relative was highest at heading stage most tissues. Among different tissues from same developmental stage, leaves, followed stems panicles. higher parent than hybrids. LC50 4.016 μg g−1, level exceeded threshold stages stems. We next lethality each strain toward After 48 h, mortality rate second-instar larvae feeding on stem tissue tiller varied 66.7 91.7%. By documenting temporal spatial evaluating risk pests diseases during production can be greatly reduced.